Serial Dilution Calculator
Calculate serial dilution concentrations step by step with a visual representation of each dilution in the series.
| Step | Concentration | Cumulative Dilution | Log(Conc) |
|---|
Each step represents a serial dilution where a portion of the previous concentration is transferred and diluted by the factor shown. Common in microbiology (CFU/mL), chemistry (standard solutions), and biochemistry (enzyme kinetics).
How to use Serial Dilution Calculator
- Enter the stock (starting) concentration and unit (e.g., 100 mg/mL).
- Enter the dilution factor using 1:10, 1/10, or just 10 format.
- Set the number of serial dilution steps to generate.
- Optionally enter transfer volume and unit for lab preparation notes.
- Click Calculate to see the full dilution table and visual chart.
What is Serial Dilution Calculator?
The Serial Dilution Calculator generates a complete dilution series from a stock solution. Enter the starting concentration, choose a dilution factor (e.g., 1:10 or 1:2), and specify the number of sequential steps. Each step dilutes the previous concentration by the given factor. The tool produces a detailed table showing concentration, cumulative dilution factor, and log(concentration) for each step, plus a visual bar chart comparing relative concentrations. Common applications include microbiology (CFU/mL enumeration), analytical chemistry (standard curve preparation), and biochemistry (enzyme assays).
FAQ
- What is a serial dilution?
- A serial dilution is a stepwise dilution of a substance in solution where each step uses the same dilution factor. For example, a 1:10 serial dilution starting at 100 mg/mL produces: 100, 10, 1, 0.1, 0.01 mg/mL. Each step takes a portion of the previous solution and dilutes it with solvent.
- How do I specify the dilution factor?
- You can enter the factor in several formats: as a ratio (1:10), as a fraction (1/10), or as plain number (10, meaning 1 part sample to 9 parts diluent). Common factors include 2 (1:2, doubling dilution), 10 (1:10, decimal dilution), and 5 (1:5).
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